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VOL. 11, ISSUE 2 (2026)
Genetic diversity analysis of tomato genotypes and varieties using RAPD markers
Authors
Jayashree M J, Asha H Sarmali, U K Kandoliya
Abstract
Assessment of genetic diversity is essential for the effective
utilization of germplasm in crop improvement programmes. The present
investigation was undertaken to evaluate the genetic diversity among twenty-two
tomato genotypes using Random Amplified Polymorphic DNA (RAPD) markers. Genomic
DNA isolated from all genotypes was amplified using twenty RAPD primers. A
total of 231 amplified bands were generated, of which 206 were polymorphic,
indicating a high level of genetic variation among the evaluated genotypes. The
average polymorphism recorded was 86.94%. The number of amplified fragments per
primer ranged from 4 to 20. Polymorphic information content (PIC) values ranged
from 0.70 to 0.93 with an average value of 0.8665, demonstrating the high
informativeness of the selected primers. Genetic similarity coefficients
estimated using Jaccard’s coefficient ranged from 0.29 to 0.85. Cluster
analysis based on the UPGMA method grouped the genotypes into two major
clusters, revealing substantial genetic divergence among the studied germplasm.
The results demonstrated the effectiveness of RAPD markers in detecting genetic
variability and identifying genetically diverse tomato genotypes that can be
utilized in future breeding programmes.
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Pages:261-264
How to cite this article:
Jayashree M J, Asha H Sarmali, U K Kandoliya "Genetic diversity analysis of tomato genotypes and varieties using RAPD markers". International Journal of Food Science and Nutrition, Vol 11, Issue 2, 2026, Pages 261-264
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