The increasing cost and limited availability of fish meal have encouraged the search for sustainable alternative protein sources in aquafeed formulation. The present study was undertaken to evaluate the proximate composition and amino acid profile of poultry by-product meal (PBM), blood meal (BM) and meat-cum-bone meal (MCBM) and to assess their suitability as partial fish meal replacers in tilapia feed. Four isonitrogenous diets were formulated, including a control diet prepared as per FAO guidelines and three treatment diets in which 50 per cent of fish meal protein was replaced with MCBM, BM or PBM. The animal by-product meals and developed feeds were analysed for proximate composition and amino acid profile. Significant variation was observed among the animal by-product meals in moisture, crude protein, ether extract, total ash, crude fibre, acid insoluble ash and nitrogen free extract. Blood meal recorded the highest crude protein content, while PBM had the highest ether extract and MCBM showed the highest ash content. The developed feeds showed comparable crude protein levels, confirming successful formulation of isonitrogenous diets. Amino acid analysis revealed that replacement of fish meal protein with animal by-product meals maintained the essential amino acid profile within a nutritionally acceptable range. Among the treatment feeds, PBM-based feed showed favourable nutrient composition and amino acid balance. The study indicated that rendered animal by-products, particularly poultry by-product meal, can be effectively utilised as alternative protein sources in tilapia feed formulation, thereby reducing dependence on fish meal and promoting sustainable aquafeed production.