This
study focused on the extraction and characterization of phenolic compounds of
Deglet Nour date seeds, as well as the evaluation of their antioxidant
activities. Three extraction solvents were compared: ethanol (80%), acetone
(50%) and water. The extraction solvent showed an effect on the studied
parameters. The highest extraction yield was obtained with ethanol (30.91%),
followed by acetone (30.72%) and water (28.92%). The total phenolic compound
content was significantly higher in the ethanolic extract (46.69 g GAE/100 g
phenolic extract), followed by the acetone (38.36 g GAE/100 g) and water (33.52
g GAE/100 g) extracts. A similar variation was observed for flavonoid concentration,
with significant differences depending on the type of solvent. Analysis by
infrared spectroscopy identified the presence of characteristic phenolic and
carbonyl compounds, with intensities varying according to the solvent used.
Antioxidant activity assessed by DPPH and ABTS tests revealed that the
ethanolic extract possessed the best activity, with respective IC₅₀ of 0.20 and
0.17 mg/mL.
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