This study Determined the Effect of Fungi Spoilage on the Nutritional Value of Corn in Langtang North Local Government Area of Plateau State. Maize also known as Corn were purchased from three different market (Sample A: Langtang North main market. Sample B: Shishiri market, Langtang North. Sample C: Kwanpe main market) located in Langtang North Local Government Area of Plateau State, and aseptically stored in labeled transparent white polythene bags. The purchased samples of Maize were transported to the Microbiology Laboratory of the National Veterinary Research Institute (NVRI) VOM, Jos Plateau State for Immediate Processing and Analysis. The results of the frequency of visible colonies shows the micro-organism isolated from the three (3) maize corn samples together with their frequency of occurrence. The Trichophyton spp was found once in samples A and none in sample B and C. Aspergillus spp also was found once in sample C and none in sample A and B. Rhizopus spp was found in sample B and C but not in sample A while Penicillium spp was found in sample B and C but not in sample A. The results of the spore formation, motility and biochemical identification of isolates shows that the Trichophyton spp was positive in carbohydrate assimilation, spore formation but negative in Amino Acid Assimilation, motility, hydrolysis and lipase activity. Aspergillus spp was positive in carbohydrate assimilation, spore formation and Amino Acid Assimilation but negative in motility, hydrolysis and lipase activity. Rhizopus spp was positive in carbohydrate assimilation, Amino Acid Assimilation and lipase activity but negative in spore formation, motility, hydrolysis. Penicillium spp was positive in carbohydrate assimilation, Amino Acid Assimilation and lipase activity but negative in spore formation, motility, hydrolysis. In conclusion, maize can be infected by fungi irrespective of storage condition. Therefore, the researcher recommended that proper and effective storage of maize through different techniques should be put in place, public awareness should be increased to sensitized people about the harmful effect of fungi on Maize so as not post harm to them.