The instability of vitamin A in edible oil has been attributed to exposure to oxygen and light, and the formation of peroxides accelerated by the same causes, and the catalytic effect of high temperature. The peroxide value and retinol retention in fortified oil at different points of the market supply chain (from factory to retailer), and during household use in Kazakhstan were evaluated. Peroxide formation and vitamin A retention were measured in oil samples stored in dark boxes and under daylight, simulating large factory (LF) and small-to-medium factory (SMF) conditions. Effect of transparent PET bottles, and an alternative opaque packaging was assessed. Both types of bottles were sealed under nitrogen. Peroxide and retinol parameters were compliant with the national regulations for safety and fortification at all points in the supply chain, regardless of exposure to light. However, after 40 days use (i.e., SMF-condition") in households did not meet safety and fortification regulations. The major inducer of oxidative deterioration and vitamin A losses was the daily opening of bottles for household use, regardless of exposure to light. Households with five or fewer members are recommended to buy 1-liter bottles rather than 5-liter bottles of oil to reduce time of use after the opening of the bottle.