Clustering analysis of Ampelopsis grossedentata based the contents of dihydromyricetin and myricetin

Fang Z Zhou; Zhi Zhou; Xiao Y Zhang; Yan K Lu; Ling Z Guo

Clustering analysis of Ampelopsis grossedentata based the contents of dihydromyricetin and myricetin

Authors
Fang Z Zhou, Zhi Zhou, Xiao Y Zhang, Yan K Lu, Ling Z Guo

Abstract
Ampelopsis grossedentata (Vine tea) has been developed as a functional tea in China. Dihydromyricetin and myricetin are the main active ingredients. However, there have been no reports about the difference of active ingredient content in vine tea produced by different manufacturers. 21 samples were collected from different enterprises. The HPLC was used to determine the contents of dihydromyricetin and myricetin in vine tea, and the Euclidean distance and group-average method were used for cluster analysis. The results showed that the content interval of dihydromyricetin and myricetin in vine tea were 168.7-349.9 mg/g and 1.3-4.9 mg/g, respectively. And clustering analysis shows that samples 3, 4, 7, 8, 10, 13, 14, 17, 20 and 21 can be grouped into one class, 12 and 6 fall into one category respectively, and the other 9 samples into a class. The main factor determining the clustering attribution is the content ratio of dihydromyricetin to myricetin in vine tea. Practical Applications: The contents of dihydromyricetin and myricetin in samples of ampelopsis grossedentata (vine tea) from 21 large-scale enterprises were determined simultaneously. The fluctuation range of dihydromyricetin and myricetin contents in vine tea was revealed. Ten vine tea samples are grouped into one class, corresponding to the first-class products reviewed by 14 experts, nine vine tea samples are grouped into another class, corresponding to the third class, and the other two samples are grouped into two categories, corresponding to the second-class products and fourth-class products. The main factor determining the clustering attribution is the content ratio of dihydromyricetin to myricetin in vine tea. These results lay the foundation for the development and application of the active ingredients based on vine tea.

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