Ameliorating effect of zinc on protein supplement induced changes in serum levels of male reproductive hormones of sprague dawley rats
Saira Safdar, Shazia Ali, Humaira Fayyaz Khan, Fozia Shamshad, Sidra Jabeen, Sana Majeed
Background: The protein supplements contain the harmful ingredients such as soy protein and silicon dioxide which have the injurious effects on male reproductive system. Addition of Zinc as an antioxidants with the dietary supplements may lessen the harmful effects of Soy protein and silicon dioxide on male reproductive system. Objectives: To determine the harmful effect Soy protein and Silicon dioxide and beneficial effect of Zinc on male reproductive parameters of Sprague Dawley rats. Material and Methods: Total 30 male Sprague Dawley rats were divided into Group 1, Group 2 and Group 3. Group 1 rats were fed on standard laboratory feed, rats of Group 2 were fed on a dietary supplement powder containing Soy protein and Silicon dioxide mixed with standard laboratory feed and rats of Group 3 were fed with a dietary supplement powder containing Soy protein, Silicon dioxide and Zinc mixed with standard laboratory feed. Serum levels of Testosterone (ng/ml), Follicle Stimulating hormone (ng/ml) and Luteinizing hormone (ng/ml) were measured by ELISA technique. Results: Group 2 rats showed significant (P<0.05) decrease in serum Testosterone level when compared with Group 1 rats. However use of Zinc in Group 3 rats significantly (P<0.05) increased serum Testosterone levels. Mean ± SEM of serum Follicle stimulating hormone (FSH) and serum Luteinizing Hormone LH levels in Group 2 rats were significantly decreased (P<0.05) as compared to Group 1.Zinc administration caused the significant increase (P<0.05) in serum Mean ± SEM of Follicle Stimulating hormone (FSH) levels and Luteinizing Hormone LH in Group 3 rats as compared to Group 1 and Group 2 rats. Conclusion: Soy protein and Silicon dioxide are found to be harmful for male reproductive system. Zinc has potential to restore normal functions of male reproductive system by increasing serum levels of Testosterone, Follicle stimulating hormone and Luteinizing hormone.