The consumption of food and beverages containing non-nutritive sugar has dramatically increased over the past few decades. There may be many reasons, this could be either growing concerns about health and quality of life to prevent overweight, obesity, cardiovascular issues and diabetes. Extensive advertisement of different company products may also attract people towards the same. Unknowingly, consumed by the consumers without the prior knowledge on these through sweets, beverages, food products etc. The main objective of this study is to understand the efficacies of Artificial Sugar Aspartame (AS) and Natural Sugar Stevia (NS) on the fitness and physiology. D. melanogaster being a better model to understand all biological process and alternative mammalian model to study toxicity, the Normal, AS and NS based food medias were used in different concentrations to grow Drosophila melanogaster flies and they were subjected to analyse, overall fitness, and physiology. Our investigation on Drosophila has likewise bolstered positive advantages of the Aspartame (AS) in few parameters and negative consequences for a few and same in Stevia (NS).