To evaluate Leucaena ecotypes native to Mexico, its phenological state and the relationship of protein fractions with in vitro dry matter digestibility (IVDMD) were evaluated using an experimental design of divided plot, large plot: ecotype (nine native); small plot: phenological stage (regrowth vs. tender green bean). Crude protein (CP), protein fractions A, B1, B2, B3 and C, and IVDMD were analysed. There was an ecotype × phenological stage effect (P < 0.05) on the protein fractions. Fraction B2 was the most abundant, and IVDMD was greater in the regrowth stage vs. green bean. IVDMD was negatively correlated with B3, C and positively correlated with CP, A, B1 and B2. Estimation IVDMD = 53.46 + 2.078 (%A) - 2.31 (%C), R² = 0.80, P < 0.05. IVDMD increased as fraction a content increase and decrease fraction C. The outstanding ecotypes are 2012-11-29, H-1, 2013-01-27, A-1 and 2013-03-01, A-6.