The objective of the present investigation was to isolate, characterize, and to identify the lactobacillus species from fruit samples banana and apple collected from local area Guntur and Mangalagiri. Fruit juices represent a promising carrier for probiotic bacteria; however, there are some drawbacks and limits that could preclude their production at the industrial level, namely the survival of probiotics throughout storage, and the possible impact of bacteria on the sensory traits and overall acceptance. Twenty Four acid producing bacteria were isolated from samples and based on morphological, biochemical and staining techniques these isolates were reduced to 14 samples. Probiotic screening was performed to these isolates acid (pH2, 3, 4) bile (0.3, 0.5%), phenol (0.2, 0.4%) and NaCl tolerance (3, 6, 9, and 12%) which are main criteria for probiotic to grow under Gut conditions. These results suggest that various Lactobacillus species are distributed in banana and apple was most abundant found in this study.