The study was undertaken with the objective of the estimated cost of herbal wine from Hibiscus Rosa-Sinenesis by using a different strain of Saccharomyces prepared by Hibiscus powder, sugar, kms, di-ammonium phosphate, and different strain MTCC178, MTCC180, MTCC786. A total three replication for experimental all the standard ingredient was purchased from the local market the result of the study were revealed that the production of cost of controlled herbal wine was lower (274/lit) compared to all herbal wine Rs. (284/lit.). Which may be attributed to higher herbal wine.