The main objective of this research is to study the effect of a different type of solvents on extraction yield and antifungal activity of Pinus pinaster needles extracts. The solvents used are: ethanol, acetone, hexane and ether. Antifungal activity was tested against four filamentous fungal strains; Aspergillus oryzae, Aspergillus nidulans, Aspergillus clavatus and Fusarium solani. The highest extraction yield was obtained by Ethanol solvent (14-21%). Acetone extracts exhibited the highest antifungal activity while hexane extracts showed the lowest one. Aspergillus oryzae and Aspergillus nidulans were the most sensitive to the extracts with inhibition rate ranging from 14.2 to 43.07% and from 0 to 34.02%, respectively.