Evaluation and method validation of sodium cyclamate in watermelon by LC-MS/MS
Dr. KJ Balasubramani, Sunitha C, Harathi C, Sivaraman KP
Watermelon is a common fruit used by most of the people in summer seasons. Sodium cyclamate is ideal for use by people suffering from diabetes. Literature shows that very less work has been done on estimation of sodium cyclamate in watermelon using LC-MS/MS. In the present study an attempt was made to develop a method for estimation of Sodium cyclamate in watermelon and is validated for its accuracy, precision, linearity, range and other validation parameters. The results showed that for the linearity the correlation coefficient was found to be 0.999, in the range of 10-1000ng/mL. The limit of detection is 1ng/mL, the limit of quantification is 3ng/mL. The RSD for six replicate injections of sodium cyclamate at 10ng/mL level were established and % RSD was found to be 3.7. Recovery was found to be 88.5% for sodium cyclamate in watermelon @ 200ng/mL.
Dr. KJ Balasubramani, Sunitha C, Harathi C, Sivaraman KP. Evaluation and method validation of sodium cyclamate in watermelon by LC-MS/MS. International Journal of Food Science and Nutrition, Volume 4, Issue 3, 2019, Pages 01-05