Various free radicals and related species such as reactive oxygen species/ROS and reactive nitrogen species/RNS were generated by various endogenously, physicochemical exposure or pathophysiological states able to alter lipids, proteins and DNA. Plants are the low-cost source of anti-oxidants with desirable nutrients. In this context, sequential solvent extracts of Vernonia anthelmintica (Kahi Jeerige/Bitter cumin) seed, was subjected to estimation phytochemical and evaluate antioxidant property. Among the extracts, aqueous Vernonia anthelmintica seed extract of (AqVA) exhibit higher levels of Phenolics, tannins, flavonoids, alkaloids and saponins followed by ethanol and water extract. The DPPH, nitric oxide, superoxide free radical scavenging activity and reducing power capacity of AVA exhibit increased activity by increased concentration. IC₅₀ values of AqVA ranged from 80.25μg/ml to 191μg/ml.