Antioxidants are gaining tremendous importance in our daily lives. These components intent to neutralize the free radicals formed in the body which are capable of damaging the body. Natural antioxidants added to foods may have a physiological impact on human health, particularly because they may reduce the risk of illnesses or they promote the delay of damaging free radicals effect, like cardiovascular diseases, intestinal cancer or infections symptoms. Synthetic antioxidants are often added to foods to retard oxidation and extend a helping hand in increasing shelf life. A variety of methods are used for determination of antioxidant activity. With the technological advances, identification of antioxidants is not a big deal. In the present article the various methods for antioxidant activity determination and for identification of antioxidants are discussed.