The plant, Gymnema lactiferum has been gradually utilized as an herbal remedy to cure various ailments, even though they have not been explored clinically. The present investigation focused mainly on the phytochemical investigation of the leaves of this plant, employing standard procedures and qualitatively and quantitatively investigation of selected phytochemicals. Results showed the presence of various phytochemicals, particularly the Alkaloids, Flavonoids, Saponins, Phenolic compounds, Protein, and Carbohydrate in Gymnema lactiferum. The quantitative test results revealed that Total Phenolic Compounds (17.2242±0.17 mg/GAE/100g), Alkaloids (0.0991±0.00187 mg/g), Saponins (0.01377±0.00532 mg/g) and IC₅₀ value for Antioxidant (9.6685 mg/ml). According to the proximate analysis; Moisture, Crude fat, Crude fiber, Crude protein, Total ash and carbohydrate contents had values of 77.5174±0.7718%, 1.0518±0.9466%, 3.2525±0.2570%, 1.9146±0.5731% and 1.9464± 0.7376% respectively. Gymnema lactiferum is a rich source for Ca, K, Na minerals. This plant contained Calcium (583.99 mg/100g), Potassium (529.28 mg/100g), Sodium (246.99 mg/100g) and Iron (14.64 mg/100g). Based on the findings of the study, Gymnema lactiferum leaves have both medicinal and nutritional potentials.