Since thousand years, the edible mushrooms are appreciated for their taste, their nutritional and therapeutic value by the populations. Besides, the trade of edible mushrooms is an important source of income for developing countries. The present work aims at improving the production of carpophore by the implementation of an adequate formulation of substratum of production of oyster mushroom. Seven substrata consisted of sawdust, its rice and some agricultural lime of variable composition were sterilized and inoculated with the spawn of 30 and 55 days old. The experimental design used is a device in two factors completely randomized with three repetitions. The parameters evaluated are the speed of growth of the mycelium on the substrata, the rate of survival primordia (TSP) and the weight of carpophores. The results showed that the substrata influence significantly the speed of growth of the mycelium. It is higher on the substrata being less than 5 % of sound of rice during the first weeks of incubation and decreases correlatively with the increasing quantities of sound of rice from the third week of incubation. The rate of survival of primordia is more important on the substratum F1 (33 %). Also the highest production was observed on the substrata F1, F2 and F3 containing less than 5 % of sound of rice.