Study of anthelmintic and Insecticidal activity of Baccaurea ramiflora plant in different extracts
Kazi Nuruddin Al Masud, Zarif Morshed, Nasiful Islam, Dr. Mahboob Hossain, Maliha Tasnim Deeba, Rezowana Islam
Baccaurea ramiflora Lour. syn. Baccaurea sapida (Roxb.) Muell. Arg. known as Burmese grapes belongs to the family Euphorbiaceae and is native to Southeast Asia. The observance on Baccaurea ramiflora leads that this plant is utilized as an antichloristic and anodyne against rheumatoid arthritis, abscesses, cellulitis and treat injuries. This plant also has anti-diarrheal, analgesic, anti-inflammatory, neuropharmacological, antioxidant and cytotoxic activities. Therefore, the present study was conducted to evaluate anthelmintic and insecticidal activities of different extract of Baccaurea ramiflora. For anthelmintic activity, Pheretima posthuma was selected as test animal while 25, 50, 100 mg/ml concentrations of samples were tested in the bioassay, from which time of paralysis and time of death of worms were estimated. Evaluation of insecticidal activity was performed against Sitophilus oryzae to calculate the mortality rate. Extracts of MEE, EE, ACE, CHE, PEE and NHE were for the activity determination. In anthelmintic study, extract exhibited its activity in dose-dependent manner showing higher the concentration, faster the effect. Extract of EE, PEE, CHE and MEE in case of 100 mg/ml concentration exhibited its paralytic effect followed by death within a short period of time among which ACE extract gave the best result which only took approx. 22.33 minutes to show paralytic effect and 33 minutes for death occurrence. In insecticidal activity, all extracts showed potent activity with 100% mortality of rice insects Sitophilus oryzae at 80 mg/ml concentration in 24hrs, proving the activity also followed dose dependent pattern. 80% mortality rate observed in case of 80 mg/ml concentration of ACE extract in 12 hr whereas MEE, CHE and NHE extracts showed 60 % and PEE showed 70% mortality rate in treated rice insects. The present study shows that all the extracts of Baccaurea ramiflora are found to possess anthelmintic and insecticidal activities.