The twelve genotypes of little millet (Panicum miliare. L) were studied for their nutritional composition and yield potential along with local check. Considerable variability was found for all the quality parameters assessed. Moisture content of seeds was found to be high in high yielding genotypes viz., TNAU-63 (11.58) OLM-20 (11.0) followed by CO-2 (10.95). Ash per cent also found to be high in high yielding genotypes such as TNAU-89 (1.21), TNAU-63 (1.15) and CO-2 (1.10) whereas, crude protein was found to be high in TNAU-89 (14.30) followed by TNAU-63 (12.99). The mean fat content, crude fibre content and carbohydrate contents were found to be higher in medium and low yielding genotypes. However, Co-2 from high yielding genotypes had recorded the highest fat content followed by OLM-23 (4.0) from medium and PRC-3 (3.90) from low yielding genotypes. Whereas, higher crude fibre content recorded in OLM-203 (5.90) from medium followed by DLM-322 (5.87) from low yielding genotypes. With respective carbohydrate content it was higher in low yielding genotypes OLM-37 (69.15) followed by DLM-322 (68.50) and Vartsukhadar (67.25).