Antioxidants and chlorophyll in Pinus pinaster Arn. needles
Sondes Fkiri, Faten Mezni, Hanene Ghazghazi, M Larbi Khouja, Abdelhamid Khaldi, Zouhaier Nasr
The aim of this study is to determine antioxidant substances, chlorophyll (A and B), carotenoids and the antioxidant activity of ethanolic extracts of needles from two Pinus pinaster varieties (var. Maghrebiana (Morocco) and var. Renoui (Tunisia)) growing in the Northwest of Tunisia. The total phenolic content was determined using Folin–Ciocalteu reagent. The total flavonoid was assessed using 2% aluminum chloride and condensed Tanins was determined with the vanillin. The antioxidant activity was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) test. Significant differences were found between the two varieties (p<0.0001).Var. Maghrebiana presented the highest polyphenol content (28.87 mg GAE/g), tannin (28 mg CE/g), Chlorophyll A (10.88 mg/L), chlorophyll B (1.06 mg/L) and carotenoids (1.22 mg/L).The most important antioxidant potential was reached by Maghrebiana variety (% inhibition DPPH= 88.53%; IC50 = 11.25 μg/mL). Renoui variety presented a lowest antioxidant activity (% inhibition DPPH = 84.97%; IC50 = 16.50 μg/mL).