The medicinal value of Indian spices has been known from centuries and they play a vital role in primary health care throughout the world. Capsicum annuum has been proposed as a promising candidate for maintaining immune system. The current investigation represents the analysis of anticoagulant and antibacterial activity of aqueous and organic (methanol, chloroform, and acetone) extracts of Capsicum annuum (Pusa jwala) by in vitro clot lysis assay and disc diffusion method respectively. The methanol chloroform, acetone and aqueous extract have shown 30.1%, 25.1%, 21.2% and 21% clot lysis respectively against control. The antibacterial assay indicated that all tested extracts exert antibacterial activities where as maximum zone of inhibition was showed by methanol extract. The qualitative phytochemical analysis revealed the presence of flavanoids, cardiac glycosides, alkaloids, phenols, saponins, resins and steroids. Thus C. annuum is suggested to have efficient potential for pharmaceutical exploitation in the treatment of cardiovascular diseases after further validation.