Milk is important part of human life. It contains vitamins those play a vital role in milk uses human consumption. Since milk is generally viewed as nutritious food with lots of vitamins, minerals and fats, proteins etc thus used for drinking. It contains vitamins those play prophylactic role in cancer, autoimmune diseases, heart diseases etc. Vitamins play a vital role in milk used for human consumption. In the present study the precipitation of casein from the various milk samples such as cow milk, goat milk, buffalo milk and also the packed milk samples available in the local market were studied. The technique of precipitation of casein is used to predict the protein content in the milk samples.