Whey based pineapple fruit juice beverages were prepared using different ratio of whey and water (90:10, 80:20, 70:30, 60:40 & 50:50), fruit juice percent (10%, 15% & 20%), sugar 10% and Citric acid 0.2% were kept constant in all the treatments. 15 treatments were prepared for chemical and sensory evaluation on an average the whey based pineapple juice beverages of treatment T₁P₁, T₁P_2, T₁P_3, T₂P_1, T₂P_3, T₃P_1, T₃P_2, T₃P_3, T₄P_1, T₄P_2, T₄P_3, T₅P_1, T₅P₂ and T₅P₃ contained moisture85.42,85.39,84.29,85.9285.87,84.76,86.43,85.31,85.28,86.98,86.94,85.88,87.51,87.47 and86.42 percent, Ash 0.32,0.32, 0.32,0.31, 0.32, 0.32, 0.28, 0.28, 0.29,0.26, 0.26, 0.27, 0.22, 0.22 and 0.23 percent, Protein 0.43,0.46,0.48,0.39, 0.42, 0.44, 0.35, 0.38, 0.40,0.31, 0.34,0.36, 0.27, 0.30 and 0.32 percent, Fat 0.40,0.41, 0.41, 0.36,0.37, 0.37,0.32, 0.33, 0.33, 0.28, 0.29, 0.29, 0.24, 0.25 and0.25 percent, Carbohydrate 13.42, 13.42,14.5, 13.01, 13.01, 14.11,12.59, 12.59, 13.7,12.17,12.17,13.2,11.76, 11.76 and 12.80 percent, Total Solid14.56, 14.61, 15.72, 14.06,14.11, 15.23,13.51,14.70,14.71,13.03,13.05,14.11,12.48,12.51 and 13.58 percent, respectively. Colour and Appearance 5.33, 5.66, 6.00, 6.33, 6.33, 5.66, 6.00, 5.33, 6.00, 6.33, 6.33, 6.33, 6.66, 6.66 and T₅P₃ 7.00, Consistency 6.00, 6.00, 6.33, 6.00, 5.66, 7.00, 7.00, 6.66, 7.00, 7.00, 6.66, 7.00, 6.00,6.66 and 7.00, Flavour and Taste 5.66,6.00, 60.66, 6.00, 6.33, 7.00, 6.00, 6.66, 7.66, 7.00, 6.00, 6.66, 6.00, 6.00 and 6.00, overall acceptability 6.00, 5.66, 6.33, 5.00, 6.00, 6.00, 5.66, 6.66, 7.666, 6.66, 7.00,7.00, 6.66,6.33 and 7.00. Whey based Pineapple fruit juice beverage prepared by using whey :water (70:30) and 20 % fruit juice T₃P₃ was found to be more acceptable as compared to sample prepared by using different combination of whey, water and fruit juice concentration, as it gave good Flavour & Taste and Overall acceptability.