Roselle plant (Hibiscus sabdariffa) of Malvaceae family finds its mention in folk medicine of North East India for its use in various ailments. In the present study, the proximate value and nutritional quality, antioxidant activity, antimicrobial properties, anti-proliferative activity and apoptosis-inducing capacity of roselle found in North East India was evaluated. Nutritional evaluation of the calyx establishes it as a good source of dietary antioxidants and ascorbic acid. Antimicrobial activity of the aqueous extract upto 25mg/ml concentration showed complete growth inhibition of both gram positive (Staph. aureus) and gram negative (E. coli, K. pneumoniae) bacteria while antioxidant activity with 100 mg/ml concentration was high (90% scavenging activity) that gradually decreases with dilution in concentration. The antiproliferative and apoptotic activities were evaluated in cervical (HeLa) cancer cell lines. It exhibited anti-proliferative activity in a concentration-dependent manner; the concentration @100mg/ml can induce upto 51.8% apoptosis. The studies indicated its potential benefits for health which can further be tested and validated for use as supplement in the food.