Walnuts are rich source of polyunsaturated fatty acid (PUFA) and highest antioxidant activity which have beneficial activity for reducing the risk of coronary heart diseases. In this study, fatty acid composition and antioxidant activity of two varieties of walnuts were investigated. The fatty acid compositions of Kashmir walnuts were analyzed by Gas chromatography. The total fat content of the sample was assessed by AOCS Official Butt-tube Method Ac 3-44 and Antioxidant activity were analyzed by radical scavenging activity (RSA) toward DPPH (2, 2-diphenyl-1-picrylhydrazyl) radical. The PUFA is highest in both the varieties (73.89%) black variety (65.74%) brown variety and MUFA is highest in brown variety (26.21%), black variety (18.17%). Brown variety shows highest radical scavenging activity compare to black variety. The present study showed that Indian Kashmir walnuts are rich in MUFA, PUFA, low in saturated fatty acid and highest antioxidant activity which might be used as a part in our diet which will reduce the risk of coronary artery diseases.