International Journal of Food Science and Nutrition

Phytochemical screening of solvent and aqueous extracts clearly showed the presence of certain important secondary metabolites in E. karvinskianus and A. adenophora. Like flavonoids, alkaloids, tannins, saponins, steroids and terpenoids. The antioxidant activity of the plant extracts were determined by using phospho-molybdenum assay, Nitric oxide free radical scavenging assay and FRAP assay. The results of the present study clearly indicated that aqueous extract showed better free radical antioxidant power assay (FRAP) in A. adenophora at concentration of 1000 mg/ml of extract. Further, the results of the nitric oxide radical scavenging assay and phosphomolybdenum assay also showed that the aqueous and ethanol extract of E. karvinskianus exhibited the highest rate of antioxidant activity.