International Journal of Food Science and Nutrition

International Journal of Food Science and Nutrition


International Journal of Food Science and Nutrition
International Journal of Food Science and Nutrition
Vol. 7, Issue 4 (2022)

GABA (γ-aminobutyric acid) promotes cell proliferation, increases MyoD and PGC-1α expression, and decreases myostatin expression in C2C12 myoblasts


Eriko Uehara, Hideki Hokazono

Multiple physiological effects of γ-aminobutyric acid (GABA) as a functional food ingredient have been reported. However, the effects of GABA on cell proliferation and the expression of myogenic determination gene number 1 (MyoD), peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α), and myostatin in C2C12 myoblasts have not been reported. In this study, we examined the effects of GABA in myoblasts. In cell counting assays, 100 µg/mL and 1000 µg/mL of GABA significantly increased cell proliferation. In real-time polymerase chain reaction and western blotting studies, GABA increased mRNA and protein expression of MyoD and PGC-1α in a concentration-dependent manner. Similar experiments also showed that GABA significantly decreased the expression of myostatin mRNA and protein in a concentration-dependent manner, confirming that GABA promotes myoblast proliferation and increases the expression of MyoD and PGC-1α, and decreases the expression of myostatin. These results suggest that GABA may increase or inhibit the decrease in muscle mass.
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How to cite this article:
Eriko Uehara, Hideki Hokazono. GABA (γ-aminobutyric acid) promotes cell proliferation, increases MyoD and PGC-1α expression, and decreases myostatin expression in C2C12 myoblasts. International Journal of Food Science and Nutrition, Volume 7, Issue 4, 2022, Pages 8-12
International Journal of Food Science and Nutrition