International Journal of Food Science and Nutrition

International Journal of Food Science and Nutrition


International Journal of Food Science and Nutrition
International Journal of Food Science and Nutrition
Vol. 4, Issue 4 (2019)

Antibiotic resistance and MAR index of Escherichia coli O157:H7 and Salmonella species isolated from smoked rat meat sold in Zaria, Nigeria


Terseer Iyene Addai

Salmonella spp. and Escherichia coli (E. coli) O157:H7 are important food-borne pathogens. This study determined the antibiotic resistance and multiple antibiotic resistance (MAR) index of Salmonella spp. and E. coli O157:H7 isolated from smoked rat meat sold in Zaria, Nigeria. A total of 384 samples were obtained for this study. The samples were analyzed in the laboratory by conventional biochemical techniques, Microgen GN-ID A+B kit for gram negative bacteria, and Rapid latex agglutination kit for E. coli O157:H7. Confirmed isolates were further subjected to antimicrobial susceptibility testing by the Agar disc diffusion technique. Results showed that both Salmonella and E. coli O157:H7 isolates exhibited multidrug resistance. Of the total (6) Salmonella isolates, 100% were resistant to tetracycline (30µg), doxycycline (30µg), amoxicillin/clavulanic acid (30µg), trimethoprim/sulfamethoxazole (25µg) and ampicillin (10 µg), 83.3% resistant to chloramphenicol (30µg) and cefixime (5µg), 66.6% resistant to gentamicin (30µg), 33.3% resistant to azithromycin (15µg), 16.6% resistant to kanamycin (30µg) and cefuroxime sodium (30µg), with no isolate (0%) resistant to ciprofloxacin (5 µg). Of the total (5) E. coli O157:H7 isolates, 100% were found to be resistant to ampicillin (10 µg) and penicillin (10 i.u), 80% resistant to streptomycin (10 µg), 60% resistant to trimethoprim/sulfamethoxazole (25 µg) and doxycycline (30µg), 40% resistant to chloramphenicol (30µg) and gentamicin (30µg), with no isolate (0%) resistant to imipenem (30µg). All isolates had MAR index of >2. The multiple antimicrobial resistance exhibited by the Salmonella and E. coli O157:H7 strains in this study is an indication of possible antibiotic abuse. Therefore, indiscriminate use of antibiotics should be discouraged.
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