Selection and optimization of bivalent solvent system for the extraction of phenolic compounds from pomegranate peel powder
Andhale Shubham, Bhoite Anjali
Present study was carried out to optimize the phenolic compound extraction of pomegranate peel in binary solvent system. ethanol ether and ethanol: water are two binary solvent systems used for extraction of phenolic compounds with potent antioxidant activity. Six different combinations of these two binary solvent systems were used for the extraction of phenolic compounds from pomegranate peel, appropriate bivalent solvent system ethanol: ether (70:30) was reported with Phenolic compounds with potent antioxidant activity (1783 mg/L of GAE phenolic content and 87.58% DPPH inhibition). In order to optimizes the extraction conditions for selected bivalent solvent system, one factor at a time study was carried out to determine the effect of time, temperature, rpm and concentration of peel powder on the total phenolic content and antioxidant activity. Studty reveals the 125 rpm at 37 oC for 6 hour is the best condition for obtaining the phenolic content with enhanced antioxidant activity for selected binary solvent system.