Alpha-linolenic acid (ALA) rich Garden cress (Lepidium sativum L.) seed oil and its vegetable oil blends modulate aggregation of platelets and serum Thromboxine B2 levels in rats
A Maheswaraiah, SS Umesha, K Akhilender Naidu
Most of the vegetables oil we consume are rich in n-6 PUFAs and deficient in n-3 PUFAs. Blending of oils is necessary to improve n-6/n-3 PUFA ratio in vegetable oils. Garden cress seeds contain 24% of oil in which 32–34% is α-linolenic Acid (ALA). Garden cress oil (GCO) is more stable due to presence of more balanced MUFA and PUFA ratio and high antioxidants in the oil. In the present study, GCO was blended with edible oils such as sunflower oil (SFO), rice bran oil (RBO), sesame oil (SESO) and flaxseed oil (FLAX), dietary feeding of GCO and its blended oils on platelet aggregation was studied Wistar rats. Dietary feeding of GCO and its blended oils significantly decreased the adenosine diphosphate (ADP) and collagen induced aggregation of platelets by 25-31% and 22-35% respectively compared to the native oils (SFO, RBO and SESO) fed rats. GCO and its blended oils fed rats showed significantly elevated ALA (5.6 and 2.6-3.1%), eicosapentaenoic acid (3.0 and 2.1-2.5%) content and decreased arachidonic acid (AA) in total fatty acids of platelets compared to native oils fed rat. Further, thromboxane B2 (TXB2)levels were decreased by 25% in SFO+GCO fed rats, 20% in RBO+GCO fed rats, 20% in SESO+GCO fed rats, and 29% in SFO+FLAX fed rats respectively compared to respective native oils fed rats. Thus, dietary feeding of n-3 polyunsaturated fatty acid (PUFA) rich GCO and its blended vegetable oils substituted AA with ALA in the platelet plasma membrane, reduced aggregation of platelets by modulating platelet thromboxane A2 in rat platelets.