Vol. 3, Issue 5 (2018)
Evaluation of chemical compositions and functional properties of bioactive compounds in Pomelo’s seeds
Author(s): Truong Ngoc Quynh Phuong, Nguyen Thi My Duyen, Phan Ngoc Hoa, Pham Van Hung, Nguyen Thi Lan Phi
Abstract: Citrus seed is a source of limonoids which brings many benefits for human health. In this study, total limonoids content of extracts from three different pomelo seeds which were grown in Vietnam including Tan Trieu seed, Thanh Tra seed, and Doan Hung seed were investigated. The limonoids were extracted using a group of three organic solvents which were ethyl acetate, acetone and methanol and then limonoids content was determined by colorimetric method using DMAB indicator reagent. The DPPH radical scavenging method was used to determine antioxidant capacity of the extracts. Moreover, anticancer of pomelo seed's extract were examined on cancer cells. The results showed that limonoid content of Tan Trieu pomelo seed was the highest (82.6 ± 0.01 µg/mg RE), followed by that of Thanh Tra pomelo seed (78.8 ± 0.02 µg/mg RE) and Doan Hung pomelo seed (66.3 ± 0.01 µg/mg RE). However, the extract of Doan Hung pomelo seed had the highest antioxidant capacity (29.6 ± 0.01%), followed by that of Thanh Tra and Tan Trieu pomelo seed (21.0 ± 0.01%). Doan Hung pomelo seed was also had significant effect on lung cancer cells with the lowest percentage in cell survival value (CS value) (67.0 ± 2.6%), followed by Thanh Tra pomelo seed (71.9 ± 1.5%) and Tan Trieu pomelo seed (87.8 ± 3.2%). In addition, Doan Hung and Tan Trieu pomelo seeds also had influence in breast cancer cells with CS values of 78.3 ± 2.7% and 79.6 ± 2.5%, respectively, whereas Thanh Tra pomelo seed had no effect on breast cancer cells. As a result, pomelo seed was considered to be a good source of bioactive compounds, antioxidants and anticancer.